Ben 10: Ultimate Challenge will be filmed in London at a themed 8,000 square-feet premises.
The show will be broadcast on Cartoon Network in the UK, Spain, France, Italy, Holland, the Nordic region, Hungary, Poland, Germany, Russia, Turkey and the Middle East.
In each version, a different person will host the action where 36 children will battle to be crowned the ultimate Ben 10 superfan.
The format will see fans answer Ben 10 general knowledge questions, perform physical and mental agility tests and take on an assault course.
Michael Carrington, chief content officer at Turner Broadcasting, said: "We’re thrilled that Ben 10 has reached a point where it merits its own spin-off show based on the popular and much-loved series. The show will be a fantastic way for Ben 10 fans across EMEA to pit their knowledge about their favourite hero against each other."
Ben 10 continues to be one of the most lucrative boys' properties around, with Turner reporting unit sales of over 500 million in EMEA alone.
Twenty Twenty chief Tim Carter commented: "Ben 10 is such a massive, creative and well-loved show for Cartoon Network, and we hope that this new format will be successful in attracting vast audiences across the territories.
"We’ve gradually built our children’s business over the past six years and this new show feels like a quantum leap forward in ambition and scale."
Twenty Twenty’s previous efforts include the reality shows Brat Camp and The Choir.