The expanded team will include three new female gladiators called Siren, Amazon and Cyclone and three male athletes -- Warrior, Doom and Goliath.
Original ITV gladiator Wolf, who took part in a "Legends" special earlier this year, will return as the gladiators' manager.
However, the original referee John Anderson, is stepping down and will be replaced by boxing referee John Coyle.
Coyle will be joined by Hugh Watkins, a rugby union referee, who will be the video referee assisting with controversial decisions.
Sky1 is expanding the production team to cope with a new schedule which plans two shows to be filmed each day.
Steve Jones, commissioning editor for Sky1, 2 and 3, said: "Our new Gladiators are an awesome sight to behold. Bigger, stronger, and even more unstoppable than before.
"We have found some brilliant characters and they will all be at the top of their game for fear of getting a rollicking from their new manager."